Table 1.
Proteomics applied in the effective mechanisms of TCM treatments in vitro.
| Diseases | CHF, CHM, and CHM compound | Targets or signaling pathways | Proteomics methods | References |
|---|---|---|---|---|
| Cancers | ||||
| Cervical carcinoma | 9,11-Dehydroergosterol peroxide | Stathmin 1 | MALDI-TOF MS/MS | [27] |
| Cervical carcinoma | Triterpenes | IL-17E, eIF5A, peroxiredoxin-2, and ubiquilin-2 | 2DE, MALDI-TOF MS/MS | [28] |
| Cervical carcinoma | Tanshinone IIA | Vimentin, Maspin, a- and b-tubulin, and GRP75 | 2DE, MALDI-TOF MS | [29] |
| Cervical carcinoma | Tanshinone IIA | Endoplasmic reticulum stress pathways | 2DE, MALDI-TOF-TOF MS | [30] |
| Hepatocellular carcinoma | 1,3,6,7-Tetrahydroxyxanthone | β-tubulin, 14-3-3σ, and P16 | 2DE, MALDI-TOF-MS, MS/MS | [31] |
| Hepatocellular carcinoma | 1,3,5-Trihydroxyxanthone | HSP27 | 2DE, MALDI-TOF-TOF MS | [32] |
| Colorectal carcinoma | Baicalein | Peroxiredoxin-6 | 2DE, MALDI-TOF-TOF MS | [33] |
| Colon carcinoma | Triptolide | 14-3-3ξ | 2DE, MALDI-TOF-TOF MS | [34] |
| Gastric adenocarcinoma | Celastrus orbiculatus | HSP27, NF-κB/Snail signal pathways | 2DE, MALDI-TOF-TOF MS | [15] |
| Gastric adenocarcinoma | Tanshinone IIA | p53, AKT, G6PI, and LDHB | iTRAQ | [35] |
| Breast carcinoma | Curcumin | TDP-43, SF2/ASF, eIF3i, 3-PGDH, and ERP29 | 2DE, MALDI-TOF MS | [14] |
| Breast carcinoma | Coptis chinensis Franch. | ROS generation | 2D-DIGE, MS | [36] |
| Leukemia | Triterpenes | Aldolase A, GAPDH, and HSP90-Alpha | 2DE, MALDI-TOF-MS | [37] |
| Cardiocerebrovascular diseases | ||||
| Heart disease | Shuanglong formula | Energy metabolism | 2DE | [38] |
| Cardiovascular disease | Salvianolic acid B | HSP27 and mitofilin | 2DE, MALDI-TOF-MS/MS | [39] |
| Alzheimer's disease | Huperzine A | Trp53 | LC-MS/MS | [40] |
| Parkinson's disease | Acanthopanax senticosus Harms | Lewy body, mitochondrial energy metabolism | iTRAQ | [41] |
| Neurodegenerative disorders | Tianma | CALR, FKBP3/4, HSP70/90, and AIP5 | iTRAQ | [42] |
| Neurodegenerative disorders | Tianma | Nxn, Dbnl, Mobkl3, Clic4, Mki67, and Bax | iTRAQ | [43] |
| Inflammatory diseases | ||||
| Inflammatory diseases | Bi-qi capsule | iNOS, COX-2, TNF-α, IL-6, and IL-1β | Proteome profiler array | [44] |
| Inflammatory diseases | Zuojin pill | iNOS, COX-2, IL-6, IL-1β, TNF-α, and NF-κB | Proteome profiler array | [45] |
| Neuroinflammation | Acanthopanax senticosus extract | Nitrosative stress pathway | 2D-DIGE, LC-ESI-MS/MS | [12] |
| Other diseases | ||||
| Wound healing | Lithospermi radix | Antioxidant activity, antiapoptosis activity | 2DE, LC-MS/MS | [46] |
| Oxidative damage | Isopsoralen | Proteins (m/z 6532 and m/z 6809) | SELDI-TOF-MS | [47] |
| Ischemia reperfusion injury | Tao Hong Si Wu decoction | Nrf2-mediated phase II enzymes | 2DE, MALDI-TOF MS | [48] |
| Postcataracts | Curcumin | Proteins (m/z of 8093 and m/z 13767) | MS | [49] |