Cooperative downregulation of STATs and NF-κB pathways in HNSCC cell lines. Ho1N1 (a, over-expresser) and HSC2 (b, baseline) cell lines were used in experiments with single and combined downregulation of STAT1, STAT3 and NF-κB subunit – RELA. Cell proliferation of Ho1N1 and HSC2 with or without of gene specific knockdown of STAT1, STAT3 and RELA was measured by CCK8. Values are mean ± SEM, the experiments were performed in pentaplicates. (* indicates a significant difference (p<0.05) between the control (scrambled) and targeted RNAi experiments.