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Jiadifenoic acids C reduces CVB3 viral proteins (VP1) synthesis in Vero cells by western blot assay. Vero cells (9×105 cells/well) were plated into 6-well culture plates for incubation for 16 h. The medium was removed and cells were infected with 600 μL CVB3 (Nancy) of 100 TCID50 for 1 h, after which various concentrations of jiadifenoic acids C were added and incubation continued for 36 h. The cells were harvested for western blot assay.
