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. 2014 Jul 16;4(4):277–283. doi: 10.1016/j.apsb.2014.06.008

Figure 6.

Figure 6

Jiadifenoic acids C reduce CVB3 viral proteins (VP1 and 3D) synthesis in Vero cells by western blot assay. Vero cells (9×105 cells/well) were plated into 6-well culture plates for incubation for 16 h. The medium was removed and cells were transfected with 4 μg plasmids pEGFP-VP1, pEGFP-VP4-VP2-VP3 (pEGFP-VP4-3), pEGFP-2C, pEGFP-3A, pEGFP-3B, pEGFP-3C and pEGFP-3D. After 48 h cells were treated with various concentrations of jiadifenoic acids C and incubation continued for 36 h. The cells were harvested for western blot assay.