Figure 4.

Hierarchy of VZV antigens stimulating poly-functional CD4⁺ T cells. (A) Grouped pie charts comparing the fold rise in poly-functional CD4⁺ T cells after stimulating PBMC with gB, gE, IE62, IE63, ORF9, and VZV lysate. Pie charts display 4+ functions (CD154⁺IFNγ⁺IL-2⁺TNFα⁺), 3+ functions (CD154⁻IFNγ⁺IL-2⁺TNFα⁺), or mono-functional cells. gB, gE, and IE62 were fractioned into multiple pools and poly-functional T cells were also assessed. Fold rise in the number of (B) 4+ (CD154⁺IFNγ⁺IL-2⁺TNFα⁺) or (C) 3+ (CD154⁻IFNγ⁺IL-2⁺TNFα⁺) CD4⁺ T cells for each VZV peptide pool. Each dot represents one subject, and the geometric mean ± 95% confidence interval is shown.