Figure 7.

ORF9-specific CD8⁺ T cells are poly-functional, produce more cytokines than mono-functional cells, and display both T_EM and T_CM phenotypes. (A) Representative gating strategy of PBMC prior to and following ZOSTAVAX vaccination that were stimulated with ORF9 peptide pool. Numbers in dot plots denotes percentages of CD8⁺ T cells producing IFNγ, IL-2, TNFα, and perforin. (B) Fold rise in the number of poly-functional CD8⁺ T cells demonstrating the heterogeneity of the T cell population. (C) The expression levels (GMFI) of IFNγ, IL-2, and TNFα by 4+ (IFNγ⁺IL-2⁺TNFα⁺perforin⁺), 3+ (IFNγ⁺IL-2⁺TNFα⁺), and mono-functional cells prior to and following ZOSTAVAX vaccination. (D) GMFI of IFNγ, IL-2, and TNFα was assessed in both ORF9-specific CD8⁺ T_CM (CCR7⁺CD45RA⁻CD45RO⁺) and T_EM (CCR7⁻CD45RA⁻CD45RO⁺).