Figure 3.

In vitro lipid digestion experiment indicating the critical process parameters that can influence the prediction of in vivo data. (1) Composition of lipid digestion media-pancreatin source, bile salts concentration, pH and volume of media and amount of lipid in the formulation. (2) Rate of calcium chloride addition-faster the rate faster the digestion. (3) pH of the media – optimize between 5.5 and 7.5. (4) Temperature – representing biological conditions at 37 °C. (5) Molarity of NaOH – low molarity leads to higher end volume of media and higher molarity leads to titrator overshooting and experimental error. (6) Stirring speed – higher speed to mix the components thoroughly.