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. 2015 Nov 2;5:15952. doi: 10.1038/srep15952

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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LMW DNA was extracted from HaCaT cells 5 days post-transfection, digested with BglI and analyzed via 2D N/N AGE. Black arrowhead, putative late theta RIs; black arrow, theta RIs; white arrows, intermediates of the second replication mechanism. 1n, monomeric (8-kbp) linear molecules; 2n, dimeric (16-kbp) linear molecules. The direction of the gel electrophoresis in the first (1D) and second (2D) dimension is indicated in the top left corner.