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. 2015 Nov 2;5:15857. doi: 10.1038/srep15857

Figure 5. Prevention of FK866-induced cardiac decompensation by NMN administration in pressure-overloaded mice.

Figure 5

(a) Kaplan-Meier survival curves of TAC-operated mice treated with FK866 (FK) + NMN (n = 9) or FK + mock (n = 8). (b) NAD+ concentrations in hearts at 7 d after operation (n = 5). (c) Sirt1 deacetylase activity in hearts at 7 d after operation (FK + NMN, n = 7; FK + mock, n = 6). (d) Immunoblot analysis of Ac-FoxO1 and FoxO1 in hearts at 7 d after operation (FK + NMN, n = 5; FK + mock, n = 5). The quantitations of the Ac-FoxO1/FoxO1 are shown as bar graphs. (e) The mRNA levels of mitochondria-associated genes in hearts at 7 d after operation (n = 4). (f) TUNEL staining (green) with nuclear staining with DAPI (blue) and WGA staining (red) showing the outlines of cardiomyocytes in mice treated with FK + NMN or FK + mock at 4 d after TAC operation. Scale bars, 40 μm. (g) Quantification of TUNEL-positive cardiomyocytes in hearts at 4 d after operation (n = 5). *P < 0.05, **P < 0.01.