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. 2015 Nov 2;5:15857. doi: 10.1038/srep15857

Figure 7. Prevention of CloLip-induced cardiac decompensation by NMN administration in pressure-overloaded mice.

Figure 7

(a) Kaplan-Meier survival curves of TAC-operated mice treated with CloLip + NMN (n = 12) or CloLip + Mock (n = 24). (b) NAD+ concentrations in hearts at 5 d after TAC operation (CloLip + NMN, n = 3; CloLip + Mock, n = 5). (c) Sirt1 deacetylase activity in hearts at 5 d after TAC operation (n = 4). (d) Immunoblot analysis of Ac-FoxO1 and FoxO1 in hearts at 5 d after TAC operation (n = 4). The quantitations of the Ac-FoxO1/FoxO1 are shown as bar graphs. (e) TUNEL staining (green) with nuclear staining with DAPI (blue) and WGA staining (red) showing the outlines of cardiomyocytes in TAC-operated mice at 2 d after operation and treatment with CloLip + NMN or CloLip + mock. Scale bars, 40 μm. (f) Quantification of TUNEL-positive cardiomyocytes in the hearts at 5 d after TAC operation (n = 5). *P < 0.05, **P < 0.01.