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. 2015 Nov 2;5:15972. doi: 10.1038/srep15972

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(a) MALAT1 siRNA significantly increased the expression of E-cadherin and inhibited the expression of N-cadherin, ZEB1, Vimentin, Slug, and Twist-1 in Tscca and Tca8113 cells determined by western blots analysis(P < 0.05). The gels were crapped, but those gels were run under the same experimental condition. (b) F-actin staining showed a stress-fiber pattern in control or NC siRNA treated cells, whereas a cortical pattern in MALAT1 siRNA-treated cells by immunofluorescence staining. (c) MALAT1 siRNA significantly upregulated E-cadherin and downregulated N-cadherin and Vimentin in Tscca and Tca8113 cells determined by immunofluorescence staining (original magnification: 1000×).