Figure 1. L-Methionine (L-Met) inhibits and disassembles PA biofilm.

(a) 24-well plate containing PA biofilm was confirmed by crystal violet staining. At different duration of incubation with or without L-Met (0.5 μM) at static condition, the culture was removed and crystal violet staining was performed and optical density was determined at 545 nm (OD₅₄₅). (b) PA was incubated with different concentrations of L-Met and at 72 h, the biomass was determined by crystal violet staining. (0.5 μM was compared with control) (c) A coverslip was placed in a 24-well plate and 72 h old biofilm was observed with Scanning electron microscopy. Scale bar: 10 μM. (d) 48 h old PA biofilm and (e) 72 h old PA biofilm were incubated with different concentrations of L-Met and the biomass was determined by crystal violet staining. (0.5 μM was compared with 0 μM). Statistical significance was calculated using One-way ANOVA. Asterisks indicate statistical significance as follows: *(p < 0.05), ***(p < 0.001).