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. 2015 Apr 7;5(3):176–187. doi: 10.1016/j.apsb.2015.03.007

Figure 1.

Figure 1

TLR2 and its endogenous ligands are up-regulated in human AAA. (A) Representative H&E staining of human AAA or control abdominal aortic tissue. (B and C) The expression of TLR2, HMGB1, HSP70 and S100A8 was detected by Western blot (n=5/group). Data are means±SD. #P<0.05, ##P<0.01, ###P<0.001, compared to control group. (D) The expression of TLR2 was detected by immunofluorescence (TLR2, FITC, green; DNA, DAPI, blue). (E) The colocalization of TLR2 and HMGB1 (TLR2, Alexa 488, green; HMGB1, Alexa 647, red; and DNA, DAPI, blue). (F and G) Blocking TLR2 attenuated the activity and expression of NF-κBp65 and HMGB1 detected by Western blot (F), and the MMP-2/9 activity in conditioned media in vitro cultured human AAA tissue detected by gelatin zymography (G). Representative images of Western blot or gelatin zymography are shown with quantitative analysis (n=5/group).