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Chemical imaging using SR-FTIR of three single microspheres (MS1–MS3). The red boxes indicate the area imaged by the IR microscope. (A) Light microscope images of PLGA microspheres; (B–E) chemical imaging for the presence of PLGA (carbonyl ester bond), PLGA (CH2 wagging), proteins (amide I) and proteins (amide II) are shown in the single microsphere.
