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. 2015 Oct 13;112(43):13372–13377. doi: 10.1073/pnas.1516473112

Fig. 3.

Fig. 3.

BIF1 and BIF4 encode Aux/IAA proteins. (A) Schematic representation of BIF1 and BIF4 genes. Exons are depicted as gray rectangles. I and II represent the EAR repressor motif and the degron domain; III/IV corresponds to the dimerization domain. (B) The amino acid sequence of the degron domains of BIF1 and BIF4 and the mutations identified. (C–J) mRNA in situ hybridizations of immature inflorescences with BIF1 and BIF4 antisense probes. Arrowheads, localized signals at the peripheral zone of the IM. (D and F) Branch meristems are shown; (H and J) spikelet meristems. (Scale bars, 100 μm.) (K) Confocal image of VENUS-BIF4 in spikelet meristems. (L) Auxin inducibility of BIF1 and BIF4. Error bars show SD. (M and N) Auxin-induced degradation profiles of normal and mutant BIF1 and BIF4 proteins.