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. 2015 Oct 13;112(43):13372–13377. doi: 10.1073/pnas.1516473112

Fig. 5.

Fig. 5.

BA1 is a target of BIF/ARF transcriptional regulatory modules. (A) Schematic showing BA1 genomic locus including 7 kb of putative promoter. Promoter fragments used as probes in EMSAs are shown as boxed regions. Values below boxes indicate position relative to BA1 start codon (+1). Gray lines indicate TGTC core AuxRE elements. (B) EMSAs show that various activating ARFs bind to BA1 promoter fragments; GST alone does not. (C) EMSA showing specificity of ARF binding to probe A. Addition of unlabeled probe A outcompetes binding to labeled probe A. (D) EMSA showing ARFs do not bind non-TGTC-enriched promoter fragments E and F. (E) Summary of protein–protein (solid lines) and protein–DNA (dashed lines) interactions identified in this study. (F) Molecular model of organogenesis in the peripheral zone of maize IMs. (G) Diagram of the resulting functional domains (false-colored). SB, suppressed bract; BD, boundary domain; AM, axillary meristem.