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. 2015 Oct 12;112(43):13348–13353. doi: 10.1073/pnas.1505271112

Fig. S7.

Fig. S7.

(A) SEC profile and Coomassie-stained SDS/PAGE (Inset) of the SAS1 variants K25A (Top), F42A (Middle), and N148G (Bottom). The arrows indicate the mass of the SEC size standard. (B) Activation of ppGpp (Left) and pppGpp (Right) synthesis of wild-type SAS1 and its variants by pppGpp. SAS1 (2 µM) was incubated in the presence of 5 mM ATP and 0.25 mM GDP or GTP without (−) or with (+) the addition of 12.5 µM pppGpp for 5 min at 37 °C and was subjected to HPLC measurement. Error bars represent the SD of three independent measurements.