Fig. 5.

Reconstitution of α-ketoacid–dependent PNR/P systems. SucCoA production by a HOAS-dependent PNR/P system depends on substrates (A) and individual enzyme components (B) in the presence of 200 μM AcCoA (C and D). AcCoA production by an AceE-dependent PNR/P system depends on substrates (E) and individual enzyme components (F). Michaelis–Menten plots and kinetic constants are described in Table 1 and SI Appendix, Figs. S14 and S16. The MS-based assay and data analysis are detailed in Materials and Methods.