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. 2015 Nov 2;16:218. doi: 10.1186/s13059-015-0784-0

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© Wong et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Structural characteristics of sgRNAs. a Secondary structure of the sgRNA. The 20-nucleotide guide sequence is complementary to the target sequence and resides at the 5′ end of the sgRNA. The highlighted nucleotides could potentially base pair, leading to an extended stem-loop structure. b Statistical significance of position-specific nucleotide accessibility of functional sgRNAs compared with non-functional sgRNAs. c Comparison of position-specific nucleotide accessibilities between functional and non-functional sgRNAs