Table 2.
FITC-labeled Nrf2 peptides of different length (8 mer to 16 mer): their binding affinities and dynamic rangea.
| Entry | Peptide name | Peptide sequence | Kd (nmol/L) | Dynamic range (ΔmA) |
|---|---|---|---|---|
| 1 | FITC-8mer Nrf2 | FITC-DEETGEFL-OH | ~750b | –c |
| 2 | FITC-8mer Nrf2-NH2 | FITC-DEETGEFL-NH2 | ~1000b | –c |
| 3 | FITC-9mer Nrf2 | FITC-LDEETGEFL-OH | 65.1±9.7 | 97.3 |
| 4 | FITC-9mer Nrf2-NH2 | FITC-LDEETGEFL-NH2 | 25.6±10.8 | 109.8 |
| 5 | FITC-10mer Nrf2 | FITC-QLDEETGEFL-OH | 30.1±6.1 | 73.5 |
| 6 | FITC-11mer Nrf2 | FITC-LQLDEETGEFL-OH | 47.7±7.4 | 96.3 |
| 7 | FITC-12mer Nrf2 | FITC-QLQLDEETGEFL-OH | 44.5±12.9 | 70.6 |
| 8 | FITC-14mer Nrf2 | FITC-FAQLQLDEETGEFL-OH | 61.9±16.5 | 64.2 |
| 9 | FITC-16mer Nrf2 | FITC-AFFAQLQLDEETGEFL-OH | 28.7±5.7 | 80.1 |
a
Anisotropy measurements were performed using FP assay106.
b
The Kd of the FITC-labeled 8mer Nrf2 peptides were assessed using the anisotropy of the fully bound FITC-9mer Nrf2 peptide.
c
Because of low binding affinity, the higher end of the dynamic range could not be determined.