Table 1.
Primers for gene cloning and real-time PCR detection.
| Primer purpose | Name | Sequence (5′–3′) |
|---|---|---|
| 5′-RACE primers | COI1-5′GSP1 | GCAAGCTCATGAAGCCATTGGCCATC |
| COI1-5′GSP2 | CCTGCAGAACACTCCCTCGCTCCCTAG | |
| 3′-RACE primers | COI1-3′GSP1 | GGAGTACGGGCTCTCCTAAGAGGTTGC |
| COI1-3′GSP2 | GCTTTGGCTGAGGGCTGCCTTGAGCTTG | |
| Full-length CDS cloing | COI1-LF | ATGGAGGAGAGCAGTTACAAG |
| COI1-LR | CCAGTTGGATCCTTTACCGTAA | |
| Reference gene primer | TUA-f | GCCAAGTGACACAAGCGTAGGT |
| TUA-r | TCCTTGCCAGAAATAAGTTGCTC | |
| AsCOI1 RT–qPCR primer | COI1-1f | CATCGTCATCGTCTTCTTCAGG |
| COI1-1r | GAGTCACATAGCCGCCCCA | |
| Universal primer A Mix (UPM) | UPM-Long | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT |
| UPM-Short | CTAATACGACTCACTATAGGGC | |
| Nested universal primer A | NUP | AAGCAGTGGTATCAACGCAGAGT |