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Effect of exposure to CG for 9 days on the BMP/WNT pathway in ST2 cells as indicated by: (A) expression of the osteoblast marker genes, BMP-2, Runx2 and β-catenin. mRNAs determined by real-time PCR analysis; (B) expression of the osteoblast markers, p-Smad1/5/8, β-catenin and Runx2. Protein expression was determined by Western blot analysis. *P<0.05, **P<0.01, ***P<0.001 versus control.
