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Effect of plasma:solvent ratio (v/v) on sensitivity. Method (A): 100 μL plasma+100 μL 50% acetonitrile; plasma:solvent (v/v) 1:1 (analyte concentration 20 μg/mL); method (B): 50 μL plasma+50 μL 50% acetonitrile+100 μL water, plasma:solvent (v/v) 1:3 (analyte concentration 10 μg/mL).
