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. Author manuscript; available in PMC: 2015 Nov 2.
Published in final edited form as: Bio Protoc. 2015 Oct 20;5(20):e1626. doi: 10.21769/bioprotoc.1626

Figure 1. Syringe Assembly.

Figure 1

Attach 2, 2mL leur-lock syringes with a stop cock at a 90° angle. Insert the plunger to the syringe perpendicular to the standing syringe. Do no insert plunger into standing syringe (A). Fill the standing syringe with the collagen working solution and CFA (B). Insert the plunger into the standing syringe and exchange the solution to the opposite syringe 20 times, until a white emulsion forms (C). Turn the syringe so leur-lock faces upward and the entire emulsion falls against a single plunger(D). Remove the stop cock and syringe without the emulsion and assemble the syringe with the 27 ½’ gauge needle (E).