Table 3.

IgG anti-citrullinated HSP90 profiles–BALF versus serum.

	citHSP90αa		citHSP90βa		PP 1C-5Cb		PP 1-5b		PP 6C-10Cb		PP 6-10b		Peptides		Tet Toxoidc
	BAL	S	BAL	S	BAL	S	BAL	S	BAL	S	BAL	S	BAL	S	BAL	S
RA-ILD
	−	−	1+	+/−	2+	−	2+	−	1+	−	1+	1+	5,5C 6, 6C 7, 7C 10,10C	5,5C 6 10Cd	4+	4+
	−	−	−	−	3+	−	1+	−	−	1+	−	1+	4C	−	+/−	3+
	−	−	−	−	1–2+	1 +	2+	2+	−	−	−	−	5,5C	5,5C	1+	3+
RA-subILD
113e	1+	NA	−	NA	−	NA	−	NA	−	NA	−	NA	NA	NA	NA	NA
	1+	2+	−	1+	1+	1+	−	−	−	−	−	−	5C	5C	−	3+
RA-no ILD
	−	−	−	−	1+	−	+/−	−	−	−	−	−	5d,5Cd	−	2+	4+
	−	−	−	−	−	−	−	−	3+	3+	−	−	6C,7C	6C,7C	−	+/−

^aResponses scored based on relative ratio of OD₄₅₀ reading compared to that of standard reference serum (with gradations corresponding to thresholds depicted in Fig. 1A (+/− = relative OD₄₅₀ 0.75–1.0, 1 + =relative OD₄₅₀ 1.0–2.0, 2+ = relative OD₄₅₀ > 2.0)), using recombinant citrullinated HSP90α or citrullinated HSP90β as substrate antigen (1 µg/ml).

^bResponses based on relative ratio of OD₄₅₀ reading compared to that of standard reference serum, using peptide pools (PP) consisting of 1C–5C, 1–5, 6C–10C, or 6–10 (5 peptides/pool, each at 0.2 µg/ml) as substrate antigens; gradations again correspond to thresholds shown in Fig. 1B (+/− = relative OD₄₅₀ 0.75–1.0, 1 + = relative OD₄₅₀ 1.0–2.0, 2+ = relative OD₄₅₀ 2.0–3.0, 3+ = relative OD₄₅₀ > 3.0).

^cRelative IgG anti-tetanus toxoid antibody titer (0 = OD₄₅₀ < 0.25, +/− = OD₄₅₀ 0.25–0.5, 1 + = OD₄₅₀ 0.5–1.0, 2+ = OD₄₅₀ 1.0–1.5, 3+ = OD₄₅₀ 1.5–2.0, 4+ = OD₄₅₀ > 2.0).

^dLow positive recognition.

^eSerum not available; NA = not assessed.

= +BALF, −serum; = +BALF, inverted serum peptide profile; = +BALF, +serum, TT_S > TT_BAL; = +BALF, +serum, TT_S = TT_BAL.