. 2013 Mar 5;3(1):21–35.

Table 4b.

Evaluation of sperm motility in different long-term extenders with CASA system

Long-term extender	Days	Concentration (x 10⁶/mL)	Motile spermatozoa (%)	Progressive spermatozoa (%)	VCL (µm/s)¹	VSL (µm/s)¹	VAP (µm/s)¹	LIN (%)¹	STR¹	AHL (µm)¹
Tri-X-Cell	0	50.59±0.73^a	91.11±1.63^a	16.66±1.38^a	102.95±2.46^a	47.23±3.02^a	65.14±5.54^a	45.87±1.24^a	0.67±0.02^a	5.96±0.47^a
	2	51.84±1.81^a	69.46±5.96^b	8.66±0.51^b	98.51±4.79^b	34.84±1.19^b	60.73±2.35^b	35.36±1.54^b	0.67±0.01^a	6.23±0.12^b
	4	50.52±1.24^a	62.34±4.53^c	5.46±0.39^c	84.94±4.66^c	25.04±0.95^c	50.55±4.01^c	29.48±2.38^c	0.66±0.01^b	6.33±0.17^b
	6	50.16±1.77^a	54.67±3.59^d	3.93±0.37^d	48.82±0.95^d	14.35±1.73^d	25.62±3.80^d	29.39±2.56^bc	0.66±0.02^b	6.41±0.41^b
	8	50.55±0.76^a	52.04±3.31^d	1.29±0.46^e	46.28±3.45^d	15.90±2.03^d	22.72±2.79^d	34.35±1.24^b	0.66±0.01^b	6.47±0.35^b
	10	49.86±0.54^a	48.39±3.24^d	1.61±0.19^e	43.72±2.33^d	10.59±2.03^e	16.91±2.20^e	24.22±3.07^b	0.64±0.01^c	6.57±0.35^b
	12	50.76±1.22^a	36.56±3.46^e	0.53±0.31^f	32.70±1.28^e	6.58±1.22^f	16.87±2.62^e	20.12±0.63^c	0.64±0.01^c	6.82±0.38^b
HP2000	0	49.77±1.84^a	91.04±1.20^a	16.50±1.12^a	84.27±5.22^a	35.62±3.33^a	47.55±2.13^a	42.27±3.11^a	0.62±0.01^a	5.56±0.23^a
	2	51.31±0.79^a	74.67±2.47^b	5.54±0.51^b	69.45±1.64^b	21.90±1.16^b	38.33±1.09^b	31.53±0.82^b	0.61±0.01^b	5.59±0.39^a
	4	50.71±1.64^a	55.88±3.54^c	2.57±0.15^c	57.20±2.08^c	15.02±0.80^c	27.21±1.64^c	26.26±2.27^b	0.60±0.02^c	5.67±0.31^a
	6	50.72±2.70^a	45.93±3.32^d	1.67±0.15^c	48.14±1.71^d	12.70±0.97^d	25.90±1.33^c	26.38±2.30^b	0.61±0.01^b	7.42±0.18^b
	8	50.99±2.24^a	44.73±4.40^d	1.57±0.20^c	35.96±4.42^e	11.21±1.63^d	20.58±1.01^d	31.17±2.43^b	0.62±0.01^a	7.45±0.38^b
	10	49.98±1.25^a	32.39±3.19^e	0.53±0.11^d	30.74±2.78^f	7.61±1.20^e	15.94±1.04^e	24.75±3.02^c	0.62±0.01^a	7.51±0.17^b
	12	51.19±0.31^a	25.63±2.49^f	0.00±0.00^e	31.94±2.98^f	6.32±0.46^e	15.82±2.08^e	19.78±2.15^c	0.60±0.01^c	7.59±0.30^b
Sus	0	51.67±0.62^a	88.43±5.32^a	16.37±0.49^a	71.91±2.19^a	34.94±3.14^a	45.64±1.30^a	48.58±2.59^a	0.61±0.02^a	5.20±0.59^a
	2	50.45±1.37^a	65.76±3.29^b	5.46±0.37^b	65.08±4.16^b	20.27±1.02^b	34.83±2.54^b	31.14±1.97^b	0.62±0.01^b	5.42±0.45^b
	4	50.73±1.11^a	44.79±3.88^c	0.50±0.10^c	57.51±1.58^c	12.63±1.21^c	26.67±2.89^c	21.96±1.49^c	0.58±0.01^c	5.61±0.42^b
	6	50.90±0.85^a	35.74±4.09^d	0.00±0.00^d	57.08±5.23^c	11.43±1.48^c	25.95±2.00^c	20.02±1.84^d	0.57±0.02^d	5.85±0.09^c
	8	49.54±1.18^a	32.62±1.21^d	0.00±0.00^d	42.72±2.19^d	9.00±0.36^d	16.92±1.08^d	21.07±0.49^d	0.57±0.01^d	5.91±0.10^c
	10	48.97±1.50^a	18.64±3.65^e	0.00±0.00^d	44.17±2.78^d	9.55±1.58^d	16.62±2.06^d	21.62±2.07^e	0.57±0.02^d	7.64±0.37^d
	12	50.78±0.56^a	14.50±2.74^e	0.00±0.00^d	23.63±4.07^e	5.13±0.21^e	9.19±1.28^e	21.71±2.94^e	0.56±0.02^e	7.64±0.36^d

^a,f Means (± SE) within a column not sharing a common superscript differ (P < 0.05).

VCL = curvilinear velocity (µm/s); VSL = straight line velocity (µm/s); VAP = average path velocity (µm/s), LIN = linearity, ratio in percentage of VSL/VCL (%); STR = straightness, VSL/VAP; ALH = amplitude of lateral head displacement (µ).