. 2013 Mar 5;3(1):21–35.

Table 5a.

Percentage of spermatozoa belong to different categories highlighted by triple staining technique.

	Short-term extender
Category	Days	MR-A 3	Androstar	Mr Pig Sire	ZM	Modena 1	BTS
Living and undamaged spermatozoa with high membrane potential	0	89.00± 5.57^a	88.00±8.19^a	88.67±2.52^a	88.00±2.00^a	88.33±2.52^a	89.67±4.04^a
	2	63.67± 1.53^a	67.67±3.51^a	65.00±3.61^a	39.67±6.03^b	50.33±1.53^c	67.33±4.93^c
	4	43.67± 1.53^a	34.00±1.00^b	40.33±3.21^c	15.33±2.52^d	20.67±3.79^e	58.33±3.06^f
	6	20.33± 4.04^a	20.67±1.53^a	19.67±3.79^a	1.67±0.58^b	15.33±2.31^c	52.67±3.51^d
Living spermatozoa with high membrane potential but acrosome reaction	0	1.00±0.00^a	0.33±0.58^a	0.00±0.00^b	1.00±0.00^a	0.67±0.58^c	1.33±0.58^d
	2	0.33±0.58^a	0.33±0.58^a	0.00±0.00^b	0.33±0.58^a	0.00±0.00^b	0.00±0.00^b
	4	1.00±1.00^a	0.33±0.58^b	0.00±0.00^c	0.00±0.00^c	0.00±0.00^c	1.00±1.00^a
	6	0.67±0.58^a	0.33±0.58^b	0.00±0.00^c	0.33±0.58^a	0.00±0.00^c	0.33±0.58^a
Living and undamaged spermatozoa with low membrane potential	0	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
	2	0.00±0.00^a	0.00±0.00^a	0.67±0.58^b	0.67±0.58^b	1.00±1.00^b	0.00±0.00^a
	4	3.00±2.65^a	0.00±0.00^b	3.33±2.08^a	1.33±0.58^c	1.33±1.15^c	1.67±0.58^c
	6	2.67±1.15^a	0.00±0.00^b	1.33±0.58^c	1.00±1.00^c	1.67±0.58^c	1.00±1.00^c
Destabilized spermatozoa with high membrane potential	0	5.33±1.53^ac	6.33±2.52^a	6.67±2.08^a	1.33±1.53^b	4.33±1.53^ac	3.33±1.53^c
	2	15.33±1.53^a	14.33±0.58^a	7.67±3.21^b	16.67±2.08^a	9.00±2.65^b	9.33±3.51^b
	4	14.33±2.08^a	13.33±1.15^ab	11.00±2.65^b	14.67±2.31^a	16.33±1.53^a	8.00±3.00^c
	6	15.67±1.53^a	14.00±3.00^a	17.00±1.73^a	14.00±2.65^a	16.33±1.53^a	9.00±4.00^b
Destabilized spermatozoa with high membrane potential and acrosome reaction	0	1.33±0.58^a	2.33±1.15^b	2.00±1.00^b	4.33±2.08^c	2.33±1.53^b	1.67±0.58^ab
	2	7.00±2.00^a	8.67±1.15^a	6.67±0.58^a	13.00±1.73^b	4.33±2.52^c	4.67±2.08^c
	4	11.33±2.08^a	10.67±1.53^a	10.00±3.46^a	14.33±2.52^b	7.67±0.58^c	7.00±2.00^c
	6	14.00±2.65^a	5.33±1.53^b	11.33±1.53^c	9.00±2.65^c	8.00±2.00^d	4.00±1.73^b

^a,f Means (± SE) within a line not sharing a common superscript differ (P < 0.05).