. 2013 Mar 5;3(1):21–35.

Table 5b.

Percentage of spermatozoa belong to different categories highlighted by triple staining technique.


Category	Days	Tri-X-Cell	HP2000	Sus
Living and undamaged spermatozoa with high membrane potential	0	87.33±4.93^a	86.00±2.00^a	83.33±6.51^a
	2	67.00±3.00^a	72.33±6.66^b	64.00±3.00^a
	4	58.33±4.04^af	54.67±3.21^a	45.33±4.04^b
	6	52.67±4.04^a	45.00±4.00^b	33.67±3.51^c
	8	50.33±2.52^a	42.33±2.08^b	32.67±1.53^c
	10	46.00±3.00^a	30.00±2.65^b	18.33±4.51^c
	12	35.67±1.15^a	22.67±2.08^b	15.33±1.53^c
Living spermatozoa with high membrane potential but acrosome reaction	0	0.33±0.58^a	0.33±0.58^a	0.00±0.00^b
	2	0.00±0.00^a	0.00±0.00^a	0.33±0.58^b
	4	0.67±0.58^a	0.00±0.00^b	0.00±0.00^b
	6	0.00±0.00^a	0.33±0.58^b	0.00±0.00^a
	8	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
	10	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
	12	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
Living and undamaged spermatozoa with low membrane potential	0	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
	2	1.33±1.15^a	0.33±0.58^b	0.00±0.00c
	4	0.00±0.00^a	1.33±1.15^b	0.00±0.00^a
	6	0.67±0.58^a	0.67±0.58^a	0.00±0.00^b
	8	0.00±0.00^a	0.00±0.00^a	0.00±0.00^a
	10	0.00±0.00^a	0.33±0.58^b	0.00±0.00^a
	12	0.00±0.00^a	0.33±0.58^b	0.00±0.00^a
Destabilized spermatozoa with high membrane potential and acrosome reaction	0	8.67±1.15^a	8.00±3.00^a	8.33±2.08^a
	2	16.67±4.04^a	13.33±1.53^a	14.33±1.53^a
	4	15.33±2.08^a	13.00±1.73^a	12.33±1.53^a
	6	15.00±1.73^a	13.67±2.52^a	12.33±1.53^a
	8	14.00±3.00^a	14.33±1.15^a	10.33±1.53^b
	10	8.67±1.15^a	15.33±2.52^b	6.67±0.58^c
	12	5.67±1.15^a	9.67±3.21^b	5.00±1.73^a
Destabilized spermatozoa with high membrane potential and acrosome reaction	0	1.00±1.00^a	1.33±0.58^a	2.00±1.73^a
	2	4.67±0.58^a	4.33±0.58^a	5.00±2.65^a
	4	6.00±2.65^a	7.67±1.15^a	8.33±1.15^a
	6	8.00±2.65^a	7.67±3.5^a	7.33±1.15^a
	8	9.00±1.00^a	9.67±2.31^a	7.67±0.58^b
	10	11.00±2.65^a	10.00±1.73^a	5.67±1.15^b
	12	4.67±1.53^a	5.33±1.53^a	5.67±1.15^a
Dead spermatozoa with low membrane potential	0	2.33±0.58^a	3.67±1.53a^b	4.33±0.58a^b
	2	7.33±1.53^a	8.67±3.06a^b	10.33±2.89a^b
	4	13.00±3.61^a	13.67±2.52^a	21.33±1.53^b
	6	16.33±1.53^a	19.67±1.15^b	30.67±3.21^c
	8	18.00±2.00^a	20.00±3.61^a	26.33±5.57^b
	10	23.00±2.00^a	26.00±2.00^b	41.33±3.06^c
	12	30.33±1.53^a	36.00±3.00^b	42.67±3.21^c
Dead spermatozoa with low membrane potential and acrosome reaction	0	0.67±0.58^a	1.33±0.58^b	2.33±0.58^c
	2	2.33±0.58^a	3.33±1.53^a	6.67±1.15^b
	4	6.67±1.15^a	10.33±2.89^b	13.33±2.08^b
	6	7.67±2.52^a	13.67±1.15^b	16.00±2.65^b
	8	9.00±1.73^a	14.67±3.06^b	23.00±2.00^c
	10	11.33±3.06^a	18.67±3.21^b	28.00±2.65^c
	12	24.67±5.13^a	26.67±2.08^b	32.00±4.00^c

^a,c Means (± SE) within a line not sharing a common superscript differ (P < 0.05).