Fig. 3.

Salmonella engulfment by Tetrahymena electroporated with RNAi corresponding to putative GPCR genes. A preliminary database search identified three putative GPCR-encoding genes (GenBank accession numbers provided in the x-axis) in Tetrahymena and siRNA was designed based on these sequences. Bacterial engulfment (average number of bacteria engulfed per cell) was determined after electroporation with siRNAs. Random RNAi and mock transformants served as controls. Data presented are the mean ± sem for three independent experiments each performed in triplicate. *p<0.05 versus the rest of the data.