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Kyte-Doolittle hydropathy plot of the putative Tetrahymena GPCR. Putative transmembrane domains were assessed using Kyte-Doolittle plots (Kyte and Doolittle, 1982) from a web-based platform (http://web.expasy.org/cgi-bin/protscale/protscale.pl). Transmembrane signatures were ascribed to peptide regions in which hydropathy scores rose from negative values to values greater than one. Putative transmembrane regions are indicated numerically from one to seven.
