Table 1.
Semi-quantitative RT-PCR of GPCR targets in Tetrahymena transformed with siRNA. Tetrahymena were transformed with siRNA and then DNA-free RNA was isolated and subjected to an RT-PCR assay that semi-quantitates transcript levels based on the number of PCR cycles required for amplicon visualization using agarose gel electrophoresis (Carlson et al., 2007).
| GenBank accession numbers corresponding to the source of the siRNA used in Tetrahymena | Number of PCR cycles required for visualization of amplicons specific to the following GenBank sequences in Tetrahymena transformed with siRNA |
||
|---|---|---|---|
| XM_ 00100979.2 | XM_ 001027519.2 | XM_ 001010055.2 | |
| XM_00100979.2 | 50** | 10 | 15 |
| XM_001027519.2 | 15 | >50** | 10 |
| XM_001010055.2 | 10 | 15 | 50** |
| random RNAi | 15 | 10 | 15 |
| mock RNAi | 10 | 10 | 10 |
**
on-target effects on transcription.