| a. 98 °C | 30 s |
| b. 98 °C | 10 s |
| c. 57 °C | 15 s |
| d. 72 °C | 2 kb/min |
| e. Repeat steps b to d | 34X |
| f. 72 °C | 5 min |
| g. 12 °C | hold |
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