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. Author manuscript; available in PMC: 2015 Nov 2.
Published in final edited form as: ACS Chem Biol. 2015 Jun 23;10(8):1831–1837. doi: 10.1021/acschembio.5b00442

Figure 3.

Figure 3

A) A study of linker length with Degradation Inducing Moiety B shows that three ethylene glycol units are optimal for the degradation of GFP-HaloTag7. B) Structures of HaloPROTACs that have weaker affinity for VHL. C) Reducing the affinity for VHL attenuates their ability to induce degradation of GFP-HaloTag7, although the effect is not necessarily linear. GFP-HaloTag7 degradation was measured by flow cytometry after 24 hour treatment with HaloPROTAC and normalized to 24 hour treatment with DMSO. All compounds were tested in triplicate except for HaloPROTAC3 (quintuplicate) and HaloPROTAC9 and HaloPROTAC10 (quadruplicate). Error bars depict the SEM.