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The primers are composed of sequences specific to the sequencing platform (green), i.e. the P5 adaptor and the Illumina primer 1 for the forward primer and the P7 adaptor and the Illumina primer 2 for the reverse primer. Downstream follows a sample identifier starting with a poly-N (red) region and the custom defined primer (blue). In the reverse primer construct, the sample identifier was replaced by a poly-N region.
