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. 2015 Nov 2;13(11):e1002290. doi: 10.1371/journal.pbio.1002290

Fig 9. IgG1+ precursors are required for constitution of the memory IgE response.

Fig 9

(A) GC B cells (B220+CD38loGL-7+; left plots) and intracellular IgE+ or IgG1+ expression on gated GC B cells (right plots) in mesenteric LN samples from day 14 N. brasiliensis-infected IgEki/ki and wild-type C57BL/6 mice. (B) PCs (B220CD138+; left plots) were gated from blasts (FSChiSSChi), and percentage of intracellular IgE+ and IgG1+ PCs is shown in the right plots. (C) Serum IgE in IgEki/ki and C57BL/6 mice after first and secondary N. brasiliensis infection. (D) Ratio of IgEb to IgEa in IgEki(a)/wt(b) or IgEwt(a)/wt(b) mice after first and secondary N. brasiliensis infection. (E) 1.5 x 105 sorted B220+IgMIgDIgE, B220+IgMIgDIgG1, B220+IgG1+ B cells or IgG1CD138+ PCs gated as indicated in S7 Fig from IgHa mice on d13 after secondary N. brasiliensis infection were transferred into nonirradiated IgHb mice. Recipient mice were infected with N. brasiliensis and IgEa was determined in the serum on day 12 after infection. Data show the mean + SEM from four independent experiments and at least seven mice (A and B), two experiments with eight mice total per group (C), two experiment with 2–7 mice (E). *p < 0.05, ***p < 0.001 by Student’s t test. n. d. = not detectable.