Figure 3.

Human lung adenocarcinoma cell lines (A549, H322, Hcc827, and H1299) and normal human embryonic lung fibroblasts HLF cell line were cultured in complete growth media, the total mRNA and protein were extracted and tested by RT-PCR and Western blot.

Notes: (A) and (B) were the result of the mRNA and protein expression of GalNAc-T3 respectively and the histogram was used as quantification of GalNAc-T3 expression in different cell lines. The expression of GalNAc-T3 was calculated relative to the β-actin expression. The data were repeated three times and are presented as the mean ± SD (^*P<0.05, ^**P≤0.001, and ^***P>0.005).

Abbreviations: RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.