. Author manuscript; available in PMC: 2016 Nov 6.

Published in final edited form as: J Mol Biol. 2015 Sep 26;427(22):3538–3553. doi: 10.1016/j.jmb.2015.09.013

Table 3.

Structural parameters and molecular-mass determination of SpeG in solution.

	SpeG ligand-free form/complex with spermine

Concentration, mg/mL	0.45		0.9		1.8		3.6		7.2
Structural parameters:	free	complex	free	complex	free	complex	free	complex	free	complex
I(0) (cm⁻¹) [from P(r)]	0.043	0.197	0.072	0.196	0.099	0.196	0.123	0.197	0.144	0.196
Rg (Å) [from P(r)]	35	45.5	36.7	42.4	38.5	42.4	39.7	42.4	40.6	42.1
I(0) (cm⁻¹) (from Guinier plot)	0.066	0.199	0.085	0.199	0.109	0.199	0.132	0.201	0.144	0.197
Rg (Å) (from Guinier plot)	34.2	43.4	36.4	43.4	38.6	43.7	39.6	43.7	39.9	43.0
D_max (Å)	117.7	147.9	125.9	140.8	131.1	134.5	131.4	125.5	139.4	121.7
Porod volume estimate (nm³)	135	436	196	445	245	448	287	455	321	448
Volume of correlation (Å³)	537	1128	660	1136	769	1139	851	1145	909	1128

Molecular-mass determination:
Molecular mass M_r [V_c²/(.1231×Rg)] (kD)	68.5	239	97.5	241	124	241	149	244	168	240
Calculated monomeric M_r from sequence (kD)	20.7