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. Author manuscript; available in PMC: 2016 Nov 2.
Published in final edited form as: Integr Biol (Camb). 2015 Nov 2;7(11):1454–1465. doi: 10.1039/c5ib00217f

Figure 7. Effect of FN fibrillogenesis and contractile force on focal adhesion size and count.

Figure 7

Cells were plated on either soft (4.3 kPa) or stiff (8.5 kPa) micropillar arrays and incubated with Y27632 or FUD. (A) Focal adhesions were imaged using antibodies against vinculin, and (B) were analysed using an author-written image processing algorithm (outlines show detected adhesions). (C) Average focal adhesion area and (D) total number of adhesions per cell were calculated for each condition. Error bars represent standard error. N > 100 focal adhesions. Scale bar = 20 microns.