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. Author manuscript; available in PMC: 2016 Nov 2.
Published in final edited form as: Integr Biol (Camb). 2015 Nov 2;7(11):1454–1465. doi: 10.1039/c5ib00217f

Figure 9. Effects of substrate stiffness and FN fibrillogenesis on nuclear morphology.

Figure 9

Changes in nucleus area, solidity and eccentricity were measured on cells grown on MPAs of increasing stiffness. (A) Solidity is defined as the ratio of nuclear area to the enclosing convex polygon area. Lower solidity indicates an invaginated nucleus (white arrows). (B) Eccentricity quantifies the elongation of the nucleus. A value closer to one is a more eccentric nucleus. (C) Nuclear area is roughly constant across stiffnesses, but is inhibited by FN fibril inhibition. (D) Eccentricity increases as a function of stiffness, and is not altered by FN inhibition. (E) Solidity increases with increasing stiffness and is inhibited on stiffer substrates. p < 0.05, N > 10 cells per condition.