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. Author manuscript; available in PMC: 2016 Nov 2.
Published in final edited form as: Inorg Chem. 2015 Oct 20;54(21):10269–10280. doi: 10.1021/acs.inorgchem.5b01576

Figure 1.

Figure 1

Long-lived Int1 intermediate formed during the H200C-HPCA reaction with O2 monitored by stopped-flow spectroscopy. H200C-HPCA (440 μM) was mixed with O2 gas-saturated buffer (1.8 mM) in 200 mM MOPS, pH 7.5 at 4 °C. Spectra were recorded between 1.26 ms and 100 s with pathlengths of 2 mm. Spectra in bold are as follows: H200C-HPCA complex, red; Int1, blue; final ring-opened product species, purple. Inset: Reduced H200C HPCD (400 μM)-HPCA (200 μM) was mixed with O2 gas-saturated buffer (1.8 mM) in 200 mM MOPS, pH 7.5 and monitored at 610 nm using a path length of 10 mm at 4 °C. Enzyme without substrate bound does not react with O2.