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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Exp Hematol. 2015 Jun 26;43(11):930–935.e6. doi: 10.1016/j.exphem.2015.06.005

Fig. 2. Genetic inactivation of Cdkn2a partially rescues genetic inactivation of Eed in MLL-AF9 cells in vivo.

Fig. 2

a) MLL-AF9/EedΔ/Δ leukemia can be established on a Cdkn2ako genetic background in vivo with incomplete penetrance and significantly prolonged latency compared to MLL-AF9/Eedff/Cdkn2ako controls. b) complete absence of H3K27me3 in MLL-AF9/EedΔ/Δ/Cdkn2ako cells in vivo (Western blot). c) Gr-1 and Mac-1 expression in vivo in MLL-AF9/Eedff/Cdkn2ako (green, gated on live GFP+ cells) and MLL-AF9/EedΔ/Δ/Cdkn2ako (yellow, gated on live GFP+YFP+ cells) blasts. d) Kit expression in vivo in MLL-AF9/Eedff/Cdkn2ako blasts (green, gated on live GFP+ cells) and MLL-AF9/EedΔ/Δ/Cdkn2ako blasts (yellow, gated on live GFP+YFP+ cells).