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. Author manuscript; available in PMC: 2016 Oct 22.
Published in final edited form as: Brain Res. 2015 Aug 10;1624:359–369. doi: 10.1016/j.brainres.2015.07.057

Fig. 5. Effect of GSNO treatment on peroxynitrite-mediated regulation of calpain activation.

Fig. 5

A. Expressions of iNOS protein were analyzed in sham, pBCCAO rats, and pBCCAO rats treated with GSNO by Western blot using specific antibody. B. Productions of peroxynitrite in the brains were analyzed in sham, pBCCAO rats, and pBCCAO rats treated with GSNO by ELISA assay as described under experimental procedure. The vertical columns are the means of individual data and T-bars are the standard error mean. *p<0.01; and as compared to sham group. +p<0.01 as compared to untreated pBCCAO group. C. The effect of increasing concentrations of GSNO or SIN-1 (a donor of peroxynitrite) on enzyme activity of calpain was analyzed by in vitro protease assay using purified calpain-1. In each reaction mixture, GSNO or SIN-1 was treated for 60 min before assay. The data points on the line represent the mean values for each individual data. ***p<0.0001 as compared to the control.