In this example, Cartoonist annotates the peak at 2327.2 Da, which is not included in the manual annotations. Cartoonist offers several alternatives for 2286.2 and 2316.2 Da, which are annotated with single cartoons in the manual annotations. Cartoonist annotates the isotope peak that it expects to be most intense; here “Iso1” means the first isotope peak, that is, one Dalton heavier than the monoisotopic form.