Figure 5.

Treatment-related differences in suppression of infection-induced gene expression. A: Plots comparing cumulative moving average changes in gene expression after Mycoplasma pulmonis infection (7 days) during treatment with vehicle, anti-angiopoietin-2 (Ang2) antibody, anti-tumor necrosis factor (TNF) antibody, or the antibody combination. Genes, ranked left to right from largest to smallest, increase after infection, have a 25% or greater increase in expression during infection, and have at least 50% suppression by one or more treatments (condition A; Table 1). Plots compare values for 100 genes (left panel) that have the greatest increases with infection and all 1006 condition A genes (right panel). Suppression by the antibody combination (red) is greater than that for anti-TNF alone (blue) or anti-Ang2 alone (green) and is also greater than the sum of values for the individual antibodies (cyan). B: Bar graphs compare the overall means for the treatment groups, both for the 100 top genes (left panel) and all 1006 genes (right panel) shown in A. Compared with the sum of the individual agents, the mean for the antibody combination is 57% greater (synergy) for the top 100 genes and 21% greater for all 1006 genes. ^∗∗∗∗P < 0.0001 for the antibody combination versus anti-Ang2 or anti-TNF given alone; ^††††P < 0.0001 versus the sum of the values for the individual agents, determined by analysis of variance and Tukey's multiple comparisons test.