Supplemental Figure S2.
Effects of reaction conditions on rates of the LRA reaction. Rates (y axis) are shown by the relative Ct values normalized to the lowest Ct within each set of conditions (1/2ΔCt). The sets of conditions evaluated are shown on the x axis and include the pH, Mg2+, and the presence of ATP with and without PA. Data are expressed as means ± SD. LRA, ligation on RNA amplification; PA, pre-adenylation.