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Chemical modifications used on the CP and the DP. Adenosine monophosphate (navy) modifications used include 1-Thio-AMP (the red denotes the sulfur on the phosphate group). Deoxyribose sugar modifications used include 2-Fluoro analogs (black). Nitrogenous base modifications used include inosine and cytosine (orange). RNA template is in lime, CP is in cyan, and DP is in pink. The 3′-OH group on DP is depicted in yellow. CP, common probe; DP, detector probe.
