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Quantitative PCR amplification curves of the detection of K103N mutant and wild-type RNA with the use of reverse transcription ASPCR. The rate of ASPCR on mutant RNA is 208 times faster than that on wild-type RNA (as determined by the difference in threshold values during quantitative PCR, 2−ΔCt). ASPCR, allele-specific PCR; NTC, no template control.
