Table 1.

List of Sequences of Oligonucleotide Probes Used for LRA, Pyrosequencing, and ASPCR Reactions

Name	Sequence
K103 wild type	5′-ACAUCCUGCAGGGUUAAAACAGAAAAAAUCAGUAACAGUACUGGAUGU-3′
K103N mutant	5′-ACAUCCUGCAGGGUUAAAACAGAACAAAUCAGUAACAGUACUGGAUGU-3′
K103N DP	5′-CACGAGTAGACACCTGCCATCCAAGCACAAGCTCTCCTGCTGAGTTACTGATC(fluoro-U)G-3′
CP	5′-/5phos/TTCTGTTTTAACCAATCGTCTTCTAGCACCACTGC/6Spacer/-3′
LRA reverse primer	5′-CACGAGTAGACACCTGCCA-3′
LRA forward primer	5′-GCAGTGGTGCTAGAAGACGA-3′
Dual-labeled probe	5′-CCAAGCACAAGCTCTCCTGCTG-3′
Pyrosequencing primer	5′-CCATCACCTACATCC-3′
Pyrosequencing amplicon	5′-TCCTGCAGGGTTAAAACAGAAAAAATCAGTAACAGTACTGGATGTAGGTGATGGAAGTACGG-3′
Pyrosequencing PCR forward primer	5′-/biotin/TCCTGCAGGGTTAAAACAGAA-3′
Pyrosequencing PCR reverse primer	5′-CCGTACTTCCATCACCTACATC-3′
ASPCR forward primer	5′-CCTACATCCAGTACTGTTACTGA-3′
ASPCR reverse primer	5′-CCTGCAGGGTTAAAACAGAAC-3′

ASPCR, allele-specific PCR; CP, common probe; DP, detector probe; LRA, ligation on RNA amplification.