Table 1.
List of target species in this work, including Enterobacteriaceae and negative control species.
| Strain | Code | Culture medium | Temperature °C | [O2] |
|---|---|---|---|---|
| Bacillus cereus | CECT 131 | BBCA | 30° | A |
| Campylobacter jejuni | ATCC 49943 | KCA | 42° | MA |
| Clostridium perfringens | CECT 376T | RCM | 37° | AN |
| Cronobacter sakazakii | ATCC BAA-894 | BESA | 37° | FAN |
| Escherichia coli DH10B | Invitrogen | EMBA | 37° | FAN |
| Listeria monocytogenes | CECT 911 | BHI, BLMA | 37° | A |
| Staphylococcus aureus | CECT 240 | MSA | 37° | FAN |
| Salmonella bongori | ATCC 43975 | KIA | 37° | FAN |
| Salmonella enterica var. arizonae | CECT 4395 | KIA | 37° | FAN |
| Salmonella enterica var. houtenae | CECT 5326 | KIA | 37° | FAN |
| Salmonella enterica var. salamanae | CECT 4000T | KIA | 37° | FAN |
| Salmonella enterica enterica var. tiphy | CECT 725 | KIA | 37° | FAN |
| Salmonella enterica enterica var. Tiphymurium | CECT 443 | KIA | 37° | FAN |
| Salmonella enterica enterica var. paratyphi | CECT 698 | KIA | 37° | FAN |
| Salmonella enterica enterica var. paratyphi | CECT 554 | KIA | 37° | FAN |
| Salmonella enterica enterica var. enteritidis | CECT 556 | KIA | 37° | FAN |
| Salmonella enterica enterica var. infantis | CECT 707 | KIA | 37° | FAN |
| Salmonella enterica enterica var. choleraesuis | CECT 724 | KIA | 37° | FAN |
| Salmonella enterica enterica var. dublin | CECT 4152 | KIA | 37° | FAN |
| Salmonella enterica enterica var. virchow | CECT 4154 | KIA | 37° | FAN |
| FAMILY ENTEROBACTERIACEAE | ||||
| Budvicia aquatica | DSM 5075 | TSA-VRBGA | 26° | FAN |
| Buttiauxella agrestis | DSM 4586 | NAI-VRBGA | 30° | FAN |
| Cedecea davisae | CECT 842T | NAI-VRBGA | 28° | FAN |
| Citrobacter youngae | CECT 5335T | LA-VRBGA | 37° | FAN |
| Edwardsiella tarda | CECT 849T | TSA-VRBGA | 26° | FAN |
| Erwinia chrysanthemi | CECT 509 | NAI-VRBGA | 37° | FAN |
| Ewingella americana | CECT 859T | TSA-VRBGA | 26° | FAN |
| Klebsiella pneumoniae | CECT 144 | TSA-VRBGA | 37° | FAN |
| Kluyvera cryocrescens | CECT 862T | NAI-VRBGA | 37° | FAN |
| Leclerciaader carboxylata | DSM 5077 | NAI-VRBGA | 37° | FAN |
| Leminorella grimontii | DSM 5078 | NAI-VRBGA | 37° | FAN |
| Morganella morganii | CECT 173T | NAI-VRBGA | 37° | FAN |
| Pantoea ananatis | CECT 4858T | TSA-VRBGA | 26° | FAN |
| Pectobacterium carotovorum | CECT 225T | TSA-VRBGA | 26° | FAN |
| Plesiomonas shigelloides | CECT 597 | NAI-VRBGA | 37° | FAN |
| Proteus vulgaris | CECT 165 | NAI-VRBGA | 37° | FAN |
| Providencia stuartii | CECT 866T | NAI-VRBGA | 37° | FAN |
| Rahnella aquatilis | DSM 4594 | NAI-VRBGA | 30° | FAN |
| Raoultella planticola | CECT 843T | NAI-VRBGA | 30° | FAN |
| Serratia marcescens | CECT 854 | TSA-VRBGA | 26° | FAN |
| Shigella dysenteriae | CECT 584 | TSA-VRBGA | 37° | FAN |
| Tatumella ptyseos | CECT 869T | TSA-VRBGA | 30° | FAN |
| Hafnia alvei | CECT 157 | TSA-VRBGA | 30° | FAN |
| Yersinia enterocolitica | CECT 4054 | TSA-VRBGA | 26° | FAN |
| NEGATIVE CONTROLS | ||||
| Bacillus badius | CECT 17T | NAI | 30° | A |
| Bacillus pumilus | CECT 29T | NAI | 30° | A |
| Bacillus subtilis | CECT 35 | NAI | 30° | A |
| Campylobacter coli | ATCC 49941 | CBA | 37° | MA |
| Campylobacter mucosalis | DSM 21682 | CBA | 37° | AN |
| Campylobacter helveticum | ATCC 51209 | CBA | 35° | MA |
| Clostridium beijerinckii | CECT 508 | LVB | 37° | AN |
| Clostridium butyricum | CECT 361T | LVB | 37° | AN |
| Enterobacter aerogenes | CECT 684T | NAI | 30° | FAN |
| Enterobacter cloacae | CECT 194T | NAI | 30° | FAN |
| Enterobacter gergoviae | CECT 857T | NAI | 37° | FAN |
| Escherichia fergusonii | DSM 13698 | NAI | 37° | FAN |
| Escherichia hermannii | DAM 4560 | NAI | 37° | FAN |
| Escherichia intermedia | ATCC 21073 | NAI | 37° | FAN |
| Listeria grayi | CECT 931T | BHI | 37° | A |
| Listeria innocua | CECT 910T | BHI | 37° | A |
| Listeria ivanovii | CECT 5379 | BHI | 37° | A |
| Staphylococcus epidermidis | CECT 231 | NAI | 37° | FAN |
| Staphylococcus haemolyticus | CECT 4900T | NAI | 37° | FAN |
| Staphylococcus simulans | CECT 4538T | NAI | 37° | FAN |
Aeration culture conditions for these strains are also indicated in next paragraph with these abbreviations: A, aerobic; AN, anaerobic; FAN, facultative anaerobic; MA, microaerophilic. Culture media for testing growth and for quantification of these strains were the following ones (see species paragraph below): BBCA, Brilliance Bacillus cereus Agar; BESA, Brilliance Enterobacter sakazakii agar; BHI, Brain-Heart Infusion; BLMA, Brilliance Listeria Agar; EMBA, Eosin Methylene Blue Agar (Levine); KCA, Karmali Campylobacter Agar; KIA, Klieger Iron Agar; LA, Luria-Bertani Agar; LVB, Liver Broth; MSA,Salt mannitol agar; NAI, Nutrient Agar I; RCM, Reinforced Clostridium Medium; VRBGA, Violet Red-Bile-Glucose Agar. For multispecies experiments, Salmonella detection and quantification was carried out using a modification of KIA, containing methylene blue (0.1 g/l), in order to avoid L. monocytogenes overgrowth on the same plates, and therefore to facilitate CFUs counting.