Table 3.
List of oligonucleotides designed for mPCR and final oligonucleotides concentrations in the mPCR.
| Primer | Sequence (5′ – 3′) | Target | Product size (bp) | Concentration |
|---|---|---|---|---|
| 1. B. cereus | ||||
| GVR-BC-Mpx-up | GCGTACTGAGTTAGAGAACGGT | cerAB | 132 | 1 μM |
| GVR-BC-Mpx-rp | TTTGCTTGCTTTGCATACGGA | (phospholipase and sphingomyelinase) | ||
| 2. C. jejuni | ||||
| GVR-CJ-Mpx-up | GAGTGAGGCGAAATTCCAAC | pldA | 251 | 2.5 μM |
| GVR-CJ-Mpx-rp | TCTCATCTCCCTTGCCATTG | (phospholipase) | ||
| 3. Cl. Perfringens | ||||
| GVR-CP-Mpx-up | TGGGAAAGTTCTTTCAACACC | rplS | 116 | 2 μM |
| GVR-CP-Mpx-rp | GAGAAAGAATCCAAGTATTCGAAGG | (ribosomal protein) | ||
| 4. Cr. sakazakii | ||||
| GVR-CS-Mpx-up | TGGCATCATCAACACTTTCGT | kpsT | 196 | 0.5 μM |
| GVR-CS-Mpx-rp | TCGACTACTACCTGGTGGACG | (capsular transport protein) | ||
| 5. E. coli | ||||
| GVR-EC-Mpx-up | GTTGGTGGGAAAGCGCGTTACA | uidA | 70 | 0.4 μM |
| GVR-EC-Mpx-rp | CGTTAAAACTGCCTGGCACAG | (β-glucuronidase) | ||
| 6. Fam. Enterobacteriaceae | ||||
| GVR-EB-Mpx-up | TCAGAGTTCCCGAAGGCACTC | 16S rRNA | 77 | 0.3 μM |
| GVR-EB-Mpx-rp | GCAACGCGAAGAACCTTACCT | |||
| 7. L. monocytogenes | ||||
| GVR-LM-Mpx-up | TGACGAAATGGCTTACAGTGA | hly | 163 | 1 μM |
| GVR-LM-Mpx-rp | GCCGAAGTTTACATTCAAGCT | (listeriolysin O) | ||
| 8. Salmonella spp. | ||||
| GVR-SE-Mpx-up | CCCGATTTTCTCTGGATGGT | invA | 176 | 0.5 μM |
| GVR-SE-Mpx-rp | GGCAATAGCGTCACCTTTGA | (invasion gene at the pathogenicity island 1) | ||
| 9. St. aureus | ||||
| GVR-SA-Mpx-up | GCAACTGAAACAACAGAAGCT | coa | 101 | 0.5 μM |
| GVR-SA-Mpx-rp | TCACGGATACCTGTACCAGCA | (staphylocoagulase) | ||